Profile
EDUCATION:
1998: Ph.D ., Molecular and Cellular Biology, Oregon State University
1991: B.A ., Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Magna cum laude , 1991
PROFESSIONAL EXPERIENCE:
2016-present: Professor, Department of Biological Sciences, University of Alberta, Edmonton, Alberta
2008-2016: Associate Professor , Department of Biological Sciences, University of Alberta, Edmonton, Alberta
2001-2008: Assistant Professor and Assistant Environmental Microbiologist , Department of Environmental Sciences, University of California, Riverside
1998-2001: Postdoctoral Scholar , Division of Geological and Planetary Sciences, California Institute of Technology and Center for Life Detection, Jet Propulsion Laboratory, NASA
1993-1998: Graduate Research Assistant, Molecular and Cellular Biology Graduate Program, Oregon State University, Corvallis
1991-1993: Professional Research Assistant, Department of Chemistry and Biochemistry, University of Colorado, Boulder
SELECT PUBLICATIONS:
Kits, K.D., C.J. Sedlacek, E.V. Lebedeva, P. Han, A. Bulaev, P. Pjevac, A. Daebeler, S. Romano, M. Albertsen, L.Y. Stein , H. Daims, M. Wagner. 2017. Kinetic analysis of a complete nitrifier reveals competitiveness in oligotrophic habitats. Nature. 549:269-272. doi: 10.1038/nature23679. Epub 2017 Aug 23.
Kozlowski, J.A., M. Stieglmeier, C. Schleper, M.G. Klotz and L.Y. Stein. 2016. Pathways and key intermediates required for obligate ammonia-dependent chemolithotrophy. ISME J. 10: 1836-1845.
Stein, L.Y. and M.G. Klotz. 2016. The Nitrogen Cycle. Curr. Biol. 26: R94–R98. http://dx.doi.org/10.1016/j.cub.2015.12.021
Kits, K.D., D.J. Campbell, A. Remus-Rosana, L.Y. Stein. Hypoxia and nitrite control denitrification activity and expression of denitrification genes in the obligate methanotroph Methylomicrobium album strain BG8. 2015. Front. Microbiol. 6:1072. doi: 10.3389/fmicb.2015.01072
Kits, K.D., M.G. Klotz and L.Y. Stein. 2015. Methane oxidation coupled to nitrate reduction under hypoxia by the gammaproteobacterium Methylomonas denitrificans sp. nov. Type Strain FJG1. Environ. Microbiol. doi: 10.1111/1462-2920.12772.
Cua, L.S. and L.Y. Stein. 2014. Characterization of denitrifying activity by the alphaproteobacterium, Sphingomonas wittichii RW1. Front. Microbiol. 5 :404. doi: 10.3389/fmicb.2014.00404
Kozlowski, J.A., J. Price and L.Y. Stein. 2014. Revision of N2 O-producing pathways in the ammonia-oxidizing bacterium, Nitrosomonas europaea ATCC 19718. Appl. Environ. Microbiol. 80 :4930-4935. doi:10.1128/AEM.01061-14
Prosser, J.I., I. Head and L.Y. Stein. 2014. The family Nitrosomonaceae. In The Prokaryotes, Alphaproteobacteria and Betaproteobacteria 4th ed. E. Rosenberg, E.F. Delong, S. Lory, E. Stackebrandt & F. Thompson (eds). Heidelberg: Springer Science. pp. 901-918
Stein, L.Y., M.A. Campbell and M.G. Klotz. 2013. Energy-mediated versus ammonium-regulated gene expression in the obligate ammonia-oxidizing bacterium, Nitrosococcus oceani . Front. Microbiol. 4 :277. doi:10.3389/fmicb. 2013.00277
Kartal, B. H.J.C.T. Wessels, E. van der Biezen, K.-J. Francoijs, M.S.M. Jetten, M.G. Klotz, and L.Y. Stein. 2012. Effects of nitrogen dioxide and anoxia on global gene and protein expression in long-term continuous cultures of Nitrosomonas eutropha C91. Appl. Environ. Microbiol. 78: 4788-4794.
SYNERGISTIC ACTIVITIES
2016: Invited participant for ASM-AGU Collaborative Colloquium on the Effects of Climate Change on Microbial Ecosystems
2015: Invited chair for Nitrification section of NSF INFEWS workshop on the Nitrogen Cycle
2015: Chair and Organizer of 4th International Conference on Nitrification and Related Processes (ICoN4)
2011-present: Member, Editorial Board, Applied and Environmental Microbiology, 2010-present: Member, Editorial Board, Environmental Microbiology
2010-2016: Specialty Chief Editor, Frontiers in Terrestrial Microbiology
2016-2017: Associate Editor, Frontiers in Microbial Physiology & Metabolism
2010-2011: Co-Editor for “Nitrification and Related Processes.” Meth. Enzymol. Vol 496
2010: Founder of Organization for Methanotroph Genome Analysis (OMeGA)
2010, 2013, 2016: US Dept. of Energy grant review panel for Systems Biology Carbon Cycling
2009-2013: Nitrification Network Steering Committee
COLLABORATORS AND OTHER AFFILIATIONS
Collaborators
Advisors:
Completed Students:
Current Students:
FES Funded Projects Outputs Show only Author
Title
Category
Date
Authors
Projects
Dr David Schindler Undergraduate Scholarship in Environmental Science AwardT01-P03 Award 2019-05-01 Malena Pintos
T01-P03 Dean's Silver Medal in Science Academic awardT01-P03 Award 2020-05-30 Malena Pintos
T01-P03 Phylogenomic analysis of the gammaproteobacterial methanotrophs (order Methylococcales) calls for the reclassification of members at the genus and species levels Orata FD, Meier-Kolthoff JP, Sauvageau D, Stein LY. 2018. Phylogenomic analysis of the gammaproteobacterial methanotrophs (order Methylococcales) calls for the reclassification of members at the genus and species levels. Frontiers in Microbiology, 9:3162.T01-P03 University of Alberta Publication 2018-12-19 T01-P03 Substrate-dependent growth variability across model methanotrophic strains Poster presentationT01-P03 University of Alberta Activity 2018-07-29 T01-P03 Introductory microbiology demonstration Lab visit and demonstration to high school studentsT01-P03 University of Alberta Activity 2018-11-28 Stein, L. , Catherine Tays,
Das, S. , Phillip Keith Sun, Fatemeh Bakhtiari Ziabari, Marc Waddingham,
" Marina Lazic
" , Hem Sharma,
" Scott Sugden
" T01-P03 Genomic taxonomy of the gammaproteobacterial methanotrophs (Methylococcales) calls for the reclassification of members within this order Poster presentationT01-P03 University of Alberta Activity 2018-07-29 T01-P03 A multi-level “-omics” approach to the study of the growth regulation of methanotrophs Poster presentationT01-P03 University of Alberta Activity 2018-10-03 T01-P03 Optimization of bacterial growth and polyhydroxybutyrate (PHB) yield by Methylocystis sp. Rockwell Poster presentationT01-P03 University of Alberta Activity 2019-03-07 T01-P03 Systematic Development of a Methanotroph chassis for the production of value-added products from C1 waste of Agriculture and Forestry: Predictive modeling and genetic engineering of Methylomicrobium album BG8 Poster presentationT01-P03 University of Alberta Activity 2019-02-20 T01-P03 Development of a Methanotroph Chassis: Genetic engineering of Methylomicrobium album BG8 for production of isoprenoids from C1 compounds Poster presentation T01-P03 University of Alberta Activity 2018-07-29 T01-P03 Development of a plasmid-based gene expression system for methanotrophs Poster presentationT01-P03 University of Alberta Activity 2019-03-08 T01-P03 Cell lysis and enhanced product recovery from methanotrophic bacteria Poster presentationT01-P03 University of Alberta Activity 2018-10-03 T01-P03 Effect of copper on the growth of methanotrophic bacteria in a fed-batch process Oral presentationT01-P03 University of Alberta Activity 2018-07-03 T01-P03 Identification and characterization of lysogenized phages in methanotrophic Poster PresentationT01-P03 University of Alberta Activity 2018-07-08 T01-P03 Identification and characterization of lysogenized phages in methanotrophic bacteria Poster presentationT01-P03 University of Alberta Activity 2018-07-29 T01-P03 Optimizing methanotroph bioproduction through growth strategies and strain adaptation Poster presentationT01-P03 University of Alberta Activity 2018-10-03 T01-P03 Adaptive Evolution of Methylomicrobium album BG8 on a low pH medium Poster presentationT01-P03 University of Alberta Activity 2019-02-20 T01-P03 The hunt for viruses that infect methane consuming bacteria Poster presentationT01-P03 University of Alberta Activity 2019-03-11 T01-P03 Combined effects of carbon and nitrogen source to optimize growth of proteobacterial methanotrophs
. published articleT01-P03 University of Alberta Publication 2018-09-25 T01-P03 The long-term relationship between microbial metabolism and greenhouse gases T01-P03 University of Alberta Publication 2020-02-12 T01-P03 Bacteriophages as a sustainable and reliable means of product recovery Oral presentationT01-P03 University of Alberta Activity 2019-05-07 T01-P03 Bioconversion of waste methane emissions to value-added biofuels and biomaterials Oral presentationT01-P03 University of Alberta Activity 2019-10-30 T01-P03 The Application of Switchable Solvents to the Extraction of Bioproducts from Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2019-05-07 T01-P03 Systematic Development of a Methanotroph Chassis for transforming wastewater methane emissions to value added products: Predictive modelling and genetic engineering of Methylomicrobium album BG8 Poster presentationT01-P03 University of Alberta Activity 2019-05-10 T01-P03 Can Pink Bacteria Help Mitigate Methane Emissions Poster presentationT01-P03 University of Alberta Activity 2020-02-20 T01-P03 Optimizing methanotroph biotechnology through growth strategies Poster presentationT01-P03 University of Alberta Activity 2019-06-11 T01-P03 Development of a nutrient supply-based process for industrial-scale mitigation of methane emissions by bacteria Poster presentationT01-P03 University of Alberta Activity 2019-06-25 T01-P03 Characterizing Outer Membrane Vesicles in Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2019-08-18 T01-P03 Isolating Outer Membrane Vesicles from Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2020-02-20 T01-P03 Optimization of growth and polyhydroxybutyrate (PHB) production from Methylocystis sp. strain Rockwell Poster presentationT01-P03 University of Alberta Activity 2019-03-09 T01-P03 Science for a Sustainable Planet Outreach presentationT01-P03 University of Alberta Activity 2019-05-08 T01-P03 Methane Bioconversions Invited presentationT01-P03 University of Alberta Activity 2019-05-28 T01-P03 Converting Fugitive Methane Emissions into Value-Added Products Oral presentationT01-P03 University of Alberta Activity 2019-06-23 T01-P03 ASM Microbe- Session co-convener Session convenerT01-P03 University of Alberta Activity 2019-06-21 T01-P03 Biomass: Microbial bioconversion to fuels and products Oral presentationT01-P03 University of Alberta Activity 2019-07-16 T01-P03 Gold College --lab training for student Outreach: Lab training workshop for studentsT01-P03 University of Alberta Activity 2019-08-20 T01-P03 Energy Solutions Outreach presentationT01-P03 University of Alberta Activity 2019-10-18 T01-P03 Microbes & Climate Change Outreach presentationT01-P03 University of Alberta Activity 2019-11-13 T01-P03 Sustainable Solutions from the Smallest Organisms Outreach presentationT01-P03 University of Alberta Activity 2020-02-01 T01-P03 USchool Hands-on demonstration OutreachT01-P03 University of Alberta Activity 2019-04-03 T01-P03 Uschool Hands-on demonstration Outreach demonstrationT01-P03 University of Alberta Activity 2020-02-03 T01-P03 Big Solutions from the Smallest Lifeforms TEDx presentationT01-P03 University of Alberta Activity 2020-03-15 T01-P03 The Application of Switchable Solvents to the Extraction of Bioproducts from Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2019-05-23 T01-P03 Improving Extraction of Biodegradable Plastics from Bacteria Poster presentationT01-P03 University of Alberta Activity 2020-02-20 T01-P03 Sea-ice algae are losing their homes and the ocean is at risk of starving Press interview with Lisa SteinT01-P03 University of Alberta Publication 2019-09-23 T01-P03 Methane-munching microbes and the carbon footprint of climate change Interview with Lisa SteinT01-P03 University of Alberta Publication 2019-11-18 T01-P03 Bioconversion of Methane to Biopolymers: An Ecofriendly and Sustainable Approach to Mitigate Environmental Problems T01-P03 University of Alberta Activity 2020-07-18 T01-P03 Bioconversion of methane to biodegradable plastic 2nd place awardT01-P03 University of Alberta Activity 2021-03-05 T01-P03 Bioconversion of methane to biodegradable plastic T01-P03 University of Alberta Activity 2020-06-25 T01-P03 Bioconversion of methane to biodegradable plastic: Metabolic route from greenhouse gas to green product Poster presentation
3rd position awardT01-P03 University of Alberta Activity 2021-03-01 T01-P03 Can pink bacteria help mitigate methane emissions? Poster presentationT01-P03 University of Alberta Activity 2020-11-23 T01-P03 Examining Protein Cargo and the Potential Physiological Mechanisms of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2021-03-05 T01-P03 The Ins and Outs of OMVs; Examining the Contents and Causes of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria Poster presentationT01-P03 University of Alberta Activity 2020-11-23 T01-P03 Metabolome profiles of the alphaproteobacterial methanotroph Methylocystis sp. Rockwell in response to carbon and nitrogen source T01-P03 University of Alberta Publication 2020-12-30 T01-P03 Transcriptomic and metabolomic responses to carbon and nitrogen sources in Methylomicrobium album BG8 T01-P03 University of Alberta Publication 2021-04-23 T01-P03 In vivo quantification of polyhydroxybutyrate (PHB ) in the alphaproteobacterial methanotroph, Methylocystis sp. Rockwell T01-P03 University of Alberta Publication 2021-12-01 T01-P03 Integrative Genome-Scale Metabolic Modeling Reveals Versatile Metabolic Strategies for Methane Utilization in Methylomicrobium album BG 8 T01-P03 University of Alberta Publication 2022-04-01 Juan C Villada, Maria F Duran, Chee Kent Lim,
Stein, L. , Patrick K H Lee
T01-P03 Climate Change Mitigation and Adaptation in Agriculture: what can biotechnology do? Rockefeller Conference on Agriculture and Climate, Part IT01-P03 University of Alberta Activity 2021-11-11 T01-P03 How Can Agrigenomics Help to Address Climate Change? Workshop on How Can Agrigenomics Help to Address Climate Change?T01-P03 University of Alberta Activity 2022-02-22 T01-P03 Chinese Assoc. for Microbial Ecology Keynote presentation for Chinese Assoc. for Microbial EcologyT01-P03 University of Alberta Activity 2022-04-28 T01-P03 Biotechnology for a Sustainable Environment Keynote talk on methane bioconversionsT01-P03 University of Alberta Activity 2021-03-21 T01-P03 Permafrost Dialogues Panel discussion on industrial solutions to thawing permafrost in Northern CanadaT01-P03 University of Alberta Activity 2021-03-15 T01-P03 Changing Methane Paradigms Workshop on methane cycling around the worldT01-P03 University of Alberta Activity 2021-02-15 T01-P03 Lysogenized phages of methanotrophic bacteria show a broad genetic diversity T01-P03 University of Alberta Activity 2021-08-05 Aurelija Grigonyte, Miranda Darlene Stahn,
" David Collins
" ,
" Marina Kalyuzhnaya
" ,
" Andrew Millard
" ,
Stein, L. ,
Sauvageau, D. T01-P03 Free Shipping? Exploring the Protein Cargo Content of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria T01-P03 University of Alberta Activity 2021-05-10 T01-P03 The Ins and Outs of OMVs; Examining the Contents and Causes of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria T01-P03 University of Alberta Activity 2021-09-22 T01-P03 Biomass production of Methylotuvimicrobium buryatense 5GB1C using two types of advanced bioreactor operations T01-P03 University of Alberta Activity 2021-09-20 T01-P03 Switchable solvents for the reversible dissolution of poly(3-hydroxybutyrate) T01-P03 University of Alberta Publication 2022-02-17 T01-P03 Transcriptomic analysis of synchrony and productivity in self-cycling fermentation of engineered yeast producing shikimic acid T01-P03 University of Alberta Publication 2021-12-03 T01-P03 Interactions of PHB and switchable hydrophilicity solvents: A more sustainable approach to PHB recovery T01-P03 University of Alberta Activity 2023-03-27 T01-P03 Adaptation of Methylobacterium extorquens AM1 to growth at low pH T01-P03 University of Alberta Activity 2023-03-16 T01-P03 In vivo and in vitro quantification of polyhydroxybutyrate (PHB) in bacteria T01-P03 University of Alberta Activity 2022-08-25 T01-P03 Interactions of PHB and switchable hydrophilicity solvents: A more sustainable approach to PHB recovery T01-P03 University of Alberta Activity 2023-03-27 T01-P03 Effect of isoprenoids on the growth of industrially relevant methanotrophs T01-P03 University of Alberta Activity 2022-08-09 T01-P03 The combined effect of methane and methanol on growth and PHB production in the alphaproteobacterial methanotroph Methylocystis sp. Rockwell. T01-P03 University of Alberta Activity 2022-08-09 T01-P03 In vivo and in vitro quantification of polyhydroxybutyrate (PHB) in bacteria T01-P03 University of Alberta Activity 2022-06-25 T01-P03 Bacteriohemerythrin at the Crossroads; Hemerythrin from Methylomonas denitrificans FJG1 sets methanotrophs apart T01-P03 University of Alberta Activity 2022-08-09 T01-P03 Advanced bioreactor operation improve methanol bioconversion in Methylotuvimicrobium buryatense 5GB1C T01-P03 University of Alberta Activity 2022-08-09 T01-P03 The influence of self-cycling fermentation long-and short-cycle schemes on Saccharomyces cerevisiae and Escherichia coli T01-P03 University of Alberta Publication 2022-08-01 T01-P03 Optimization of Methane Feed and N:C Ratio for Biomass and Polyhydroxybutyrate Production by the Alphaproteobacterial Methanotroph Methylocystis sp. Rockwell T01-P03 University of Alberta Publication 2022-12-06 T01-P03 An overlooked influence of reactive oxygen species on ammonia-oxidizing microbial communities in redox-fluctuating aquifers T01-P03 University of Alberta Publication 2023-04-15 " Helin Wang
" ,
" Ping Li
" ,
" Xiaohan Liu
" ,
" Jing Zhang
" ,
Stein, L. ,
" Ji-Dong Gu
" T01-P03 Microbiome of the freshwater sponge Ephydatia muelleri shares compositional and functional similarities with marine sponges T01-P03 University of Alberta Publication 2022-07-29 " Scott Sugden
" ,
" Johannes Holert
" ,
" Erick Erick Erick Erick Cardenas, William W Mohn,
Stein, L. T01-P03 Positron-emitting tracers spatially resolve unexpected biogeochemical relationships linked with methane oxidation in Arctic soils T01-P03 University of Alberta Publication 2022-04-04 Michael P Schmidt, Steven D Mamet,
" Curtis Senger
" ,
" Alixandra Schebel
" ,
" Mitsuaki Ota
" , Tony W Tian,
" Umair Aziz
" ,
Stein, L. ,
" Tom Regier
" ,
" Kevin Stanley
" ,
" Derek Peak
" , Steven D Siciliano
T01-P03 The double life of Methanoperedens T01-P03 University of Alberta Publication 2023-01-12 T01-P03 Microbial methane munchers offer a shield from the scorch T01-P03 University of Alberta Publication 2023-09-14 T01-P03 Methanol bioconversion in Methylotuvimicrobium buryatense 5GB1C through self-cycling fermentation Methanol is an abundant and low-cost next-generation carbon source. While many species of methanotrophic bacteria can convert methanol into valuable bioproducts in bioreactors, Methylotuvimicrobium buryatense 5GB1C stands out as one of the most promising strains for industrialization. It has a short doubling time compared to most methanotrophs, remarkable resilience against contamination, and a suite of tools enabling genetic engineering. When approaching industrial applications, growing M. buryatense 5GB1C on methanol using common batch reactor operation has important limitations; for example methanol toxicity leads to mediocre biomass productivity. Advanced bioreactor operation strategies, such as fed-batch and self-cycling fermentation, have the potential to greatly improve the industrial prospects of methanotrophs growing on methanol. Herein, implementation of fed-batch operation led to a 26-fold increase in biomass density, while two different self-cycling fermentation (SCF) strategies led to 3-fold and 10-fold increases in volumetric biomass productivity. Interestingly, while synchronization is a typical trait of microbial populations undergoing SCF, M. buryatense 5GB1C cultures growing under this mode of operation led to stable, reproducible cycles but no significant synchronization.T01-P03 University of Alberta Publication 2023-05-09 T01-P03 Diverse bacteriohemerythrin genes of Methylomonas denitrificans FJG1 provide insight into the survival and activity of proteobacterial methanotrophs under hypoxia PreprintT01-P03 University of Alberta Publication 2024-01-07 T01-P03 Lysogenized phages of methanotrophic bacteria show a broad and untapped genetic diversity preprintT01-P03 University of Alberta Publication 2022-05-21 Miranda Darlene Stahn, Aurelija Grigonyte, Fabini Orata, David A Collins, Liam Rieder, Marina G Kalyuzhnaya,
" Andrew Millard
" ,
Stein, L. ,
Sauvageau, D. T01-P03
